Table 3.

Comparison of fluorescent substrates’ kinetics, assay time, quality, and cost.

Fluorescent substrate	BFC	BOMCC	DBF	DBOMF	RBE	7BQ
Apparent Km (µM) (95% CI)	9.96 (2.27–44.8)	23.9 (18.5–31.4)	0.525 (0.247–1.16)	1.62 (1.06–2.44)	6.74 (3.74–14.9)	0.913 (0.379–1.97)
Apparent Vmax (pmol/min/pmol CYP3A7) (95% CI)	0.0896 (0.0578–0.172)	0.369 (0.328–0.423)	0.148 (0.114–0.204)	7.85 (7.02–8.79)	0.0825 (0.0597–0.139)	1.30 (1.08–1.56)
Total assay time (min)	60	55	105	70	90	50
Relative cost per plate ($)	72.58	125.81	70.96	78.84	75.35	71.15
S.N. ratio	87.4	349	222	381	96.4	41.9
Z′ factor	0.845	0.918	0.889	0.874	0.746	0.726