Table 2.
The affinity and number of binding sites for ORF3a and its complexes with porphyrins in relation to chlorin according to the results of direct and reverse titration.
| Complex | Scatchard constant | Number of binding sites | Type of titration |
|---|---|---|---|
| ORF3a * Chlorin | 1.04 × 106 | 1.1 | UV–Vis, reverse |
| 1.07 × 106 | 0.5 | Fluorescence, reverse | |
| 7.1 × 105 | 0.9 | UV–Vis, direct | |
| 4.6 × 104 | 1.7 | Fluorescence, direct | |
| ORF3a*PP | 7.9 × 104 | 1.0 | UV–Vis, direct |
| 7.5 × 104 | 2.0 | Fluorescence, direct | |
| ORF3a*ClFePP | 4.68 × 105 | 2.5 | UV–Vis, direct |
| 8.3 × 104 | 2.7 | Fluorescence, direct |