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. 2021 Oct 1;8:209. doi: 10.1038/s41438-021-00644-6

Fig. 5. PpNAC.A59 directly interacts with the PpERF.A16 promoter.

Fig. 5

A PpNAC.A59-activated LUC expression, which was controlled by PpERF.A16 promoter. B Y1H assay of PpNAC.A59 with seven fragments (I to VII) in the PpERF.A16 promoter. C Kinetic assay and EMSA of PpNAC.A59 with the PpERF.A16 promoter. “+” and “−” indicate the presence and absence of recombinant PpNAC.A59::HIS, biotin-labeled probe, or cold probe, respectively. Cold probe concentrations were tenfold (10×) and 100-fold (100×) those of the labeled probes