. 2021 Sep 30;11:19429. doi: 10.1038/s41598-021-98582-9

Table 3.

Mitigation of secondary stress response (catalase and superoxide dismutase) through dietary supplementation of selenium nanoparticles, eicosapentanoic acid and dicosahexanoic acid fed to P. hypophthalmus reared in control or under arsenic and high temperature for 105 days.

Treatments	Non-stressors	Stressors (arsenic and temperature)	Non-stressors			Stressors (arsenic and temperature)			P-value
Diets	Control	Control	Se-NPs + EPA + DHA-0.2%	Se-NPs + EPA + DHA-0.4%	Se-NPs + EPA + DHA-0.6%	Se-NPs + EPA + DHA-0.2%	Se-NPs + EPA + DHA-0.4%	Se-NPs + EPA + DHA-0.6%	P-value
CAT-Liver	3.96^d ± 0.26	6.70f. ± 0.61	2.29^c ± 0.19	0.91^a ± 0.13	4.24^e ± 0.31	2.13^c ± 0.24	1.29^b ± 0.11	6.39f. ± 0.18	0.0023
CAT-Gill	3.11^c ± 0.40	8.44^d ± 0.61	1.61^b ± 0.25	0.78^a ± 0.08	3.04c ± 0.53	1.52b ± 0.28	0.81a ± 0.07	3.21^c ± 0.59	0.014
CAT-Kidney	5.73^c ± 0.76	9.01^d ± 0.38	2.68^b ± 0.29	1.04^a ± 0.15	5.09^c ± 0.41	2.22^b ± 0.31	1.20^a ± 0.19	9.47^d ± 0.92	0.002
SOD-Liver	66.0^b ± 1.82	75.04^d ± 1.14	66.71^b ± 1.16	62.02^a ± 1.13	70.85^c ± 1.09	66.75^b ± 1.50	62.59^a ± 0.58	72.07^c ± 0.91	0.034
SOD-Gill	31.49^a ± 1.23	38.71^b ± 0.58	30.63^a ± 1.44	32.01^a ± 1.40	30.31^a ± 1.84	32.26^a ± 0.80	30.15^a ± 1.96	30.84^a ± 1.46	0.027
SOD-Kidney	41.16^a ± 1.29	46.66^b ± 2.16	42.32^a ± 1.54	40.57^a ± 0.98	43.34^ab ± 1.25	40.97^a ± 0.95	41.0^a ± 1.52	47.15^b ± 1.46	0.043

Values in the same row with different superscript (a, b, c, d, e, f) differ significantly. Data expressed as Mean ± SE (n = 6). Catalase and SOD: Units/mg protein.