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. 1999 Nov;19(11):7801–7815. doi: 10.1128/mcb.19.11.7801

FIG. 2.

FIG. 2

A msh2 mutation rescues the synthetic lethality between pol30-104 and rad52. (a and b) pol30-104 (RDKY3583), pol30-104 msh2 (RDKY3584), pol30-104 rad52 (RDKY3585) and pol30-104 msh2 rad52 (RDKY3586) strains bearing a URA3 POL30 plasmid were grown at 30°C in liquid SD −Ura to saturation; 10-fold serial dilutions of each culture were spotted onto an SD −Ura plate to determine viability (a) and an SD +5FOA plate to determine whether the URA3 POL30 plasmid was required for viability (b). (c and d) pol30-104 msh2 (RDKY3669) and three independent isolates of pol30-104 msh2 rad52 (RDKY3587), all bearing URA3 POL30 and TRP1 MSH2 plasmids, were grown at 30°C in liquid SD −Ura −Trp media to saturation; 10-fold serial dilutions of each culture were spotted onto an SD −Ura −Trp plate to determine viability (c) and an SD −Trp +5FOA plate to determine whether the URA3 POL30 plasmid was required for viability in the presence of the TRP1 MSH2 plasmid (d).