TABLE 1.
S. cerevisiae strains used in this study
| Strain | Genotype |
|---|---|
| RDKY3023a | MATa ura3-52 leu2Δ1 trp1Δ63 his3Δ200 lys2ΔBgl hom3-10 ade2Δ1 ade8 |
| RDKY2706 | MATa ura3-52 leu2Δ1 trp1Δ63 his3Δ200 lys2ΔBgl hom3-10 ade2Δ1 ade8 MSH2::hisG |
| RDKY3545 | MATa ura3-52 leu2Δ1 trp1Δ63 his3Δ200 lys2ΔBgl hom3-10 ade2Δ1 ade8 [URA3 POL30] |
| RDKY3546 | MATa ura3-52 leu2Δ1 trp1Δ63 his3Δ200 lys2ΔBgl hom3-10 ade2Δ1 ade8 [TRP1 pol30-41] |
| RDKY3547 | MATa ura3-52 leu2Δ1 trp1Δ63 his3Δ200 lys2ΔBgl hom3-10 ade2Δ1 ade8 [TRP1 pol30-45] |
| RDKY3548 | MATa ura3-52 leu2Δ1 trp1Δ63 his3Δ200 lys2ΔBgl hom3-10 ade2Δ1 ade8 [TRP1 pol30-52] |
| RDKY3549 | MATa ura3-52 leu2Δ1 trp1Δ63 his3Δ200 lys2ΔBgl hom3-10 ade2Δ1 ade8 POL30::hisG-URA3-hisG [TRP1 pol30-41] |
| RDKY3550 | MATa ura3-52 leu2Δ1 trp1Δ63 his3Δ200 lys2ΔBgl hom3-10 ade2Δ1 ade8 POL30::hisG-URA3-hisG[TRP1 pol30-45] |
| RDKY3551 | MATa ura3-52 leu2Δ1 trp1Δ63 his3Δ200 lys2ΔBgl hom3-10 ade2Δ1 ade8 POL30::hisG-URA3-hisG[TRP1 pol30-52] |
| RDKY3552 | MATa ura3-52 leu2Δ1 trp1Δ63 his3Δ200 lys2ΔBgl hom3-10 ade2Δ1 ade8 LEU2.POL30 |
| RDKY3553 | MATa ura3-52 leu2Δ1 trp1Δ63 his3Δ200 lys2ΔBgl hom3-10 ade2Δ1 ade8 LEU2.pol30-100 |
| RDKY3554 | MATa ura3-52 leu2Δ1 trp1Δ63 his3Δ200 lys2ΔBgl hom3-10 ade2Δ1 ade8 LEU2.pol30-102 |
| RDKY3555 | MATa ura3-52 leu2Δ1 trp1Δ63 his3Δ200 lys2ΔBgl hom3-10 ade2Δ1 ade8 LEU2.pol30-103 |
| RDKY3556 | MATa ura3-52 leu2Δ1 trp1Δ63 his3Δ200 lys2ΔBgl hom3-10 ade2Δ1 ade8 LEU2.pol30-104 |
| RDKY3557 | MATa ura3-52 leu2Δ1 trp1Δ63 his3Δ200 lys2ΔBgl hom3-10 ade2Δ1 ade8 LEU2.pol30-105 |
| RDKY3558 | MATa ura3-52 leu2Δ1 trp1Δ63 his3Δ200 lys2ΔBgl hom3-10 ade2Δ1 ade8 LEU2.pol30-106 |
| RDKY3559 | MATa ura3-52 leu2Δ1 trp1Δ63 his3Δ200 lys2ΔBgl hom3-10 ade2Δ1 ade8 LEU2.pol30-108 |
| RDKY3560b | MATa ura3-52 leu2Δ1 trp1Δ63 his3Δ200 lys2ΔBgl hom3-10 ade2Δ1 ade8 LEU2.pol30-126 |
| RDKY3561 | MATa ura3-52 leu2Δ1 trp1Δ63 his3Δ200 lys2ΔBgl hom3-10 ade2Δ1 ade8 LEU2.pol30-114 |
| RDKY3543 | MATa ura3-52 leu2Δ1 trp1Δ63 his3Δ200 lys2ΔBgl hom3-10 ade2Δ1 ade8 LEU2.POL30 msh2::hisG-URA3-hisG |
| RDKY3562 | MATa ura3-52 leu2Δ1 trp1Δ63 his3Δ200 lys2ΔBgl hom3-10 ade2Δ1 ade8 LEU2.pol30-100 msh2::hisG-URA3-hisG |
| RDKY3563 | MATa ura3-52 leu2Δ1 trp1Δ63 his3Δ200 lys2ΔBgl hom3-10 ade2Δ1 ade8 LEU2.pol30-102 msh2::hisG-URA3-hisG |
| RDKY3564 | MATa ura3-52 leu2Δ1 trp1Δ63 his3Δ200 lys2ΔBgl hom3-10 ade2Δ1 ade8 LEU2.pol30-103 msh2::hisG-URA3-hisG |
| RDKY3565 | MATa ura3-52 leu2Δ1 trp1Δ63 his3Δ200 lys2ΔBgl hom3-10 ade2Δ1 ade8 LEU2.pol30-104 msh2::hisG-URA3-hisG |
| RDKY3566 | MATa ura3-52 leu2Δ1 trp1Δ63 his3Δ200 lys2ΔBgl hom3-10 ade2Δ1 ade8 LEU2.pol30-105 msh2::hisG-URA3-hisG |
| RDKY3567 | MATa ura3-52 leu2Δ1 trp1Δ63 his3Δ200 lys2ΔBgl hom3-10 ade2Δ1 ade8 LEU2.pol30-108 msh2::hisG-URA3-hisG |
| RDKY3568b | MATa ura3-52 leu2Δ1 trp1Δ63 his3Δ200 lys2ΔBgl hom3-10 ade2Δ1 ade8 LEU2.pol30-126 msh2::hisG-URA3-hisG |
| RDKY3569 | MATa ura3-52 leu2Δ1 trp1Δ63 his3Δ200 lys2ΔBgl hom3-10 ade2Δ1 ade8 LEU2.pol30-114 msh2::hisG-URA3-hisG |
| RDKY3544 | MATa ura3-52 leu2Δ1 trp1Δ63 his3Δ200 lys2ΔBgl hom3-10 ade2Δ1 ade8 LEU2.POL30 msh6::hisG-URA3-hisG |
| RDKY3570 | MATa ura3-52 leu2Δ1 trp1Δ63 his3Δ200 lys2ΔBgl hom3-10 ade2Δ1 ade8 LEU2.pol30-100 msh6::hisG-URA3-hisG |
| RDKY3571 | MATa ura3-52 leu2Δ1 trp1Δ63 his3Δ200 lys2ΔBgl hom3-10 ade2Δ1 ade8 LEU2.pol30-102 msh6::hisG-URA3-hisG |
| RDKY3572 | MATa ura3-52 leu2Δ1 trp1Δ63 his3Δ200 lys2ΔBgl hom3-10 ade2Δ1 ade8 LEU2.pol30-103 msh6::hisG-URA3-hisG |
| RDKY3573 | MATa ura3-52 leu2Δ1 trp1Δ63 his3Δ200 lys2ΔBgl hom3-10 ade2Δ1 ade8 LEU2.pol30-104 msh6::hisG-URA3-hisG |
| RDKY3574 | MATa ura3-52 leu2Δ1 trp1Δ63 his3Δ200 lys2ΔBgl hom3-10 ade2Δ1 ade8 LEU2.pol30-105 msh6::hisG-URA3-hisG |
| RDKY3575 | MATa ura3-52 leu2Δ1 trp1Δ63 his3Δ200 lys2ΔBgl hom3-10 ade2Δ1 ade8 LEU2.pol30-108 msh6::hisG-URA3-hisG |
| RDKY3576b | MATa ura3-52 leu2Δ1 trp1Δ63 his3Δ200 lys2ΔBgl hom3-10 ade2Δ1 ade8 LEU2.pol30-126 msh6::hisG-URA3-hisG |
| RDKY3577 | MATa ura3-52 leu2Δ1 trp1Δ63 his3Δ200 lys2ΔBgl hom3-10 ade2Δ1 ade8 LEU2.pol30-114 msh6::hisG-URA3-hisG |
| RDKY3578b | MATa ura3-52 leu2Δ1 trp1Δ63 his3Δ200 lys2ΔBgl hom3-10 ade2Δ1 ade8 LEU2.pol30-126 [pRDK762 (URA3 GAL-RAD27)] |
| RDKY3588b | MATa ura3-52 leu2Δ1 trp1Δ63 his3Δ200 lys2ΔBgl hom3-10 ade2Δ1 ade8 LEU2.pol30-126 [pCH1071 (URA3 GAL)] |
| RDKY3668 | MATa ura3-52 leu2Δ1 trp1Δ63 his3Δ200 lys2ΔBgl hom3-10 ade2Δ1 ade8 LEU2.POL30 [pRDK762 (URA3 GAL-RAD27)] |
| RDKY3579 | MATa ura3-52 leu2 LEU2.POL30 |
| MATα leu2 can1-51 hom3 | |
| RDKY3580 | MATa ura3-52 leu2 LEU2.POL30 MSH2::G418 |
| MATα leu2 can1-51 hom3 MSH2::G418 | |
| RDKY3581 | MATa ura3-52 leu2 LEU2 pol30-104 |
| MATα leu2 can1-51 hom3 LEU2 pol30-104 | |
| RDKY3582 | MATa ura3-52 leu2 LEU2 pol30-104 MSH2::G418 |
| MATα leu2 can1-51 hom3 LEU2 pol30-104 MSH2::G418 | |
| RDKY3583 | MATa ura3-52 leu2Δ1 trp1Δ63 his3Δ200 lys2ΔBgl hom3-10 ade2Δ1 ade8 LEU2.pol30-104 [pCH1511 (URA3 POL30)] |
| RDKY3584 | MATa ura3-52 leu2Δ1 trp1Δ63 his3Δ200 lys2ΔBgl hom3-10 ade2Δ1 ade8 LEU2.pol30-104 msh2::HisG [pCH1511 (URA3 POL30)] |
| RDKY3585 | MATa ura3-52 leu2Δ1 trp1Δ63 his3Δ200 lys2ΔBgl hom3-10 ade2Δ1 ade8 LEU2.pol30-104 rad52::His3 [pCH1511 (URA3 POL30)] |
| RDKY3586 | MATa ura3-52 leu2Δ1 trp1Δ63 his3Δ200 lys2ΔBgl hom3-10 ade2Δ1 ade8 LEU2.pol30-104 rad52::His3 msh2::hisG [pCH1511 (URA3 POL30)] |
| RDKY3587 | MATa ura3-52 leu2Δ1 trp1Δ63 his3Δ200 lys2ΔBgl hom3-10 ade2Δ1 ade8 LEU2.pol30-104 rad52::his3 msh2::hisG [pCH1511(URA3 POL30)] [pRDK447 (TRP1 MSH2)] |
| RDKY3669 | MATa ura3-52 leu2Δ1 trp1Δ63 his3Δ200 lys2ΔBgl hom3-10 ade2Δ1 ade8 LEU2.pol30-104 msh2::hisG [pCH1511 (URA3 POL30)] [pRDK447 (TRP1 MSH2)] |
a
The nature of the ade8 mutation in RDKY3023 was previously undetermined. In the course of this study, we determined that the ade8 mutation resulted from the deletion of a CT dinucleotide at positions 104 and 105 (where the A of the initiating methionine codon is nucleotide +1).
b
pol30-112 was originally described as a mutation that changed nucleotide 719 from an A to a G (2). However, on resequencing the original pol30-112 plasmid, we found that the originally described mutation was present along with a second T→A change at nucleotide position 443, resulting in a V→D change at amino acid position 148. Thus, the original pol30-112 allele was a double mutant. When this double mutation was transferred to the strains constructed here by gene replacement, only the N-terminal T→A change at nucleotide position 443 was inherited and was responsible for the phenotypes observed. Because this resulted in the creation of a new allele, we have named this allele pol30-126 in accordance with the numbering used in reference 2.