TABLE 2.
Mutation rate analysis
| Genotype | RDKY no. | Group(s) | Mutation ratea
|
||
|---|---|---|---|---|---|
| CAN1 | hom3-10 | lys2-bgl | |||
| Set a (pol30 mutants) | |||||
| POL30 | 3552 | 3.6 × 10−7 (1) | 1.0 × 10−8 (1) | 1.7 × 10−8 (1) | |
| POL30/POL30 | 3545 | 3.3 × 10−7 (1) | 1.2 × 10−8 (1) | 1.9 × 10−8 (1) | |
| pol30-41/POL30 | 3546 | 6.5 × 10−7 (2) | n.d. | 6.9 × 10−8 (3) | |
| pol30-45/POL30 | 3547 | 2.2 × 10−7 (1) | n.d. | 1.8 × 10−8 (1) | |
| pol30-52/POL30 | 3548 | 11 × 10−7 (3) | 57 × 10−8 (57) | 63 × 10−8 (37) | |
| pol30-41 | 3549 | 6.4 × 10−7 (2) | 2.7 × 10−8 (3) | 4.1 × 10−8 (2) | |
| pol30-45 | 3550 | B | 4.8 × 10−7 (1) | 1.5 × 10−8 (2) | 3.4 × 10−8 (2) |
| pol30-52 | 3551 | A | 55 × 10−7 (15) | 243 × 10−8 (243) | 70 × 10−8 (41) |
| pol30-100 | 3553 | C | 16 × 10−7 (4) | 16 × 10−8 (16) | 8.4 × 10−8 (5) |
| pol30-102 | 3554 | 6.8 × 10−7 (2) | 2.2 × 10−8 (2) | 2.8 × 10−8 (2) | |
| pol30-103 | 3555 | B, C | 13 × 10−7 (4) | 2.8 × 10−8 (3) | 6.6 × 10−8 (4) |
| pol30-104 | 3556 | A | 94 × 10−7 (26) | 324 × 10−8 (324) | 35 × 10−8 (21) |
| pol30-105 | 3557 | B, C | 6.8 × 10−7 (2) | 1.5 × 10−8 (2) | 3.3 × 10−8 (2) |
| pol30-106 | 3558 | 3.6 × 10−7 (1) | 0.9 × 10−8 (1) | 1.6 × 10−8 (1) | |
| pol30-108 | 3559 | A, C | 19 × 10−7 (5) | 15 × 10−8 (15) | 6.4 × 10−8 (4) |
| pol30-126 | 3560 | A, B | 22 × 10−7 (6) | 19 × 10−8 (19) | 8.6 × 10−8 (5) |
| pol30-114 | 3561 | B, C | 7.7 × 10−7 (2) | 6.0 × 10−8 (6) | 3.8 × 10−8 (2) |
| msh2 | 3543 | 64 × 10−7 (18) | 500 × 10−8 (500) | 140 × 10−8 (82) | |
| Set b (pol30 msh2 double mutants) | |||||
| msh2 | 3543 | 97 × 10−7 (27) | 800 × 10−8 (800) | 144 × 10−8 (85) | |
| msh2 pol30-100 | 3562 | C | 153 × 10−7 (43) | 742 × 10−8 (742) | 157 × 10−8 (92) |
| msh2 pol30-102 | 3563 | 75 × 10−7 (20) | 860 × 10−8 (860) | 134 × 10−8 (79) | |
| msh2 pol30-103 | 3564 | B, C | 200 × 10−7 (54) | 3,363 × 10−8 (3,363) | 663 × 10−8 (390) |
| msh2 pol30-104 | 3565 | A | 310 × 10−7 (84) | 1,700 × 10−8 (1,700) | 251 × 10−8 (148) |
| msh2 pol30-105 | 3566 | B, C | 127 × 10−7 (34) | 740 × 10−8 (740) | 155 × 10−8 (91) |
| msh2 pol30-108 | 3567 | A, C | 297 × 10−7 (80) | 1,567 × 10−8 (1,567) | 274 × 10−8 (161) |
| msh2 pol30-126 | 3568 | A, B | 97 × 10−7 (27) | 1,077 × 10−8 (877) | 140 × 10−8 (82) |
| msh2 pol30-114 | 3569 | B, C | 98 × 10−7 (27) | 985 × 10−8 (985) | 129 × 10−8 (76) |
| Set c (pol30 msh6 double mutants) | |||||
| msh6 | 3544 | 21 × 10−7 (6) | 5.8 × 10−8 (6) | 2.6 × 10−8 (2) | |
| msh6 pol30-100 | 3570 | C | 90 × 10−7 (25) | 44 × 10−8 (44) | 16 × 10−8 (9) |
| msh6 pol30-102 | 3571 | 20 × 10−7 (6) | 4.5 × 10−8 (5) | 2.2 × 10−8 (1) | |
| msh6 pol30-103 | 3572 | B, C | 84 × 10−7 (23) | 17 × 10−8 (17) | 15 × 10−8 (9) |
| msh6 pol30-104 | 3573 | A | 252 × 10−7 (70) | 879 × 10−8 (879) | 122 × 10−8 (72) |
| msh6 pol30-105 | 3574 | B, C | 48 × 10−7 (13) | 3.8 × 10−8 (4) | 2.8 × 10−8 (2) |
| msh6 pol30-108 | 3575 | A, C | 131 × 10−7 (36) | 56 × 10−8 (56) | 27 × 10−8 (16) |
| msh6 pol30-126 | 3576 | A, B | 55 × 10−7 (15) | 37 × 10−8 (37) | 11 × 10−8 (6) |
| msh6 pol30-114 | 3577 | B, C | 36 × 10−7 (10) | 32 × 10−8 (32) | 9 × 10−8 (5) |
| Set d (presence or absence of RAD27 overexpression) | |||||
| wt | 3552 | ND | ND | 1.0 × 10−8 (1) | |
| wt (GAL-RAD27) | 3668 | ND | ND | 1.8 × 10−8 (2) | |
| pol30-126 | 3588 | A, B | ND | ND | 6.5 × 10−8 (7) |
| pol30-126 (GAL-RAD27) | 3578 | A, B | ND | ND | 5.6 × 10−8 (6) |
a
Average of two or more experiments. Numbers in parentheses indicate fold induction relative to the wild-type (wt) mean value. pol30 msh2 or pol30 msh6 double mutants that are significantly higher than those for corresponding msh2, msh6, or pol30 single mutants (Mann-Whitney test, P < 0.05) are in boldface. ND, not determined.