TABLE 3.
CAN1 mutation spectra for various pol30 mutants
| Genotype | Type of event | Frequency (%) | Mutationa |
|---|---|---|---|
| pol30-41 | |||
| Gross deletions | 0/22 (0) | ||
| Gross insertions | 0/22 (0) | ||
| Frameshifts | 7/22 (32) | ||
| 1/22 | G1→G0 | ||
| 1/22 | G2→G3 | ||
| 1/22 | G3→G2 | ||
| 1/22 | C1→C0 | ||
| 1/22 | T4→T3 | ||
| 1/22 | T6→T7 | ||
| 1/22 | T2→T1 | ||
| Base substitution | 15/22 (68) | ||
| 1/22 | G→T | ||
| 1/22 | G→A | ||
| 1/22 | G→C | ||
| 4/22 | C→T | ||
| 4/22 | C→G | ||
| 1/22 | C→A | ||
| 1/22 | T→G | ||
| 2/22 | A→T | ||
| Complex events | 0/21 (0) | ||
| pol30-45 | |||
| Gross deletions | 1/23 (4) | 39 bp1 | |
| Gross insertions | 1/23 (4) | 38 bp2 | |
| Frameshifts | 1/23 (4) | C1→C0 | |
| Base substitution | 20/23 (88) | ||
| 1/23 | C→T | ||
| 2/23 | C→A | ||
| 3/23 | C→G | ||
| 3/23 | G→A | ||
| 4/23 | G→C | ||
| 2/23 | G→T | ||
| 1/23 | T→A | ||
| 1/23 | T→G | ||
| 1/23 | T→C | ||
| 1/23 | A→T | ||
| 1/23 | A→G | ||
| Complex events | 0/23 (0) | ||
| pol30-52 | |||
| Gross deletions | 0/20 (0) | ||
| Gross insertions | 0/20 (0) | ||
| Frameshifts | 16/20 (80) | ||
| 2/20 | G4→G3 | ||
| 1/20 | G0→G1 | ||
| 1/20 | A3→A2 | ||
| 5/20 | A6→A5 | ||
| 1/20 | A2→A3 | ||
| 1/20 | A6→A7 | ||
| 1/20 | T5→T4 | ||
| 1/20 | T3→T2 | ||
| 1/20 | T4→T3 | ||
| 2/20 | T6→T7 | ||
| Base substitution | 4/20 (20) | ||
| 1/20 | C→T | ||
| 1/20 | T→C | ||
| 1/20 | A→T | ||
| 1/20 | A→G | ||
| Complex events | 0/20 (0) | ||
| pol30-100 | |||
| Gross deletions | 0/21 (0) | ||
| Gross insertions | 0/21 (0) | ||
| Frameshifts | 2/21 (10) | ||
| 1/21 | T6→T7 | ||
| 1/21 | G2→G1 | ||
| Base substitution | 19/21 (90) | ||
| 2/21 | G→T | ||
| 3/21 | G→A | ||
| 2/21 | G→C | ||
| 1/21 | C→T | ||
| 2/21 | C→G | ||
| 4/21 | C→A | ||
| 1/21 | T→G | ||
| 1/21 | T→A | ||
| 1/21 | A→G | ||
| 1/21 | A→C | ||
| Complex events | 0/21 (0) | ||
| pol30-102 | |||
| Gross deletions | 0/29 (0) | ||
| Gross insertions | 0/29 (0) | ||
| Frameshifts | 9/29 (31) | ||
| 3/29 | T2→T1 | ||
| 2/29 | T1→T0 | ||
| 1/29 | T3→T4 | ||
| 1/29 | T6→T5 | ||
| 2/29 | C3→C2 | ||
| Base substitution | 20/29 (69) | ||
| 5/29 | G→A | ||
| 3/29 | G→T | ||
| 6/29 | C→A | ||
| 3/29 | C→T | ||
| 1/29 | T→G | ||
| 1/29 | T→C | ||
| 1/29 | A→T | ||
| Complex events | 0/29 (0) | ||
| pol30-103 | |||
| Gross deletions | 4/20 (20) | 60 bp3 | |
| 40 bp4 | |||
| 49 bp5 | |||
| 41 bp6 | |||
| Gross insertions | 0/20 (0) | ||
| Frameshifts | 5/20 (25) | ||
| 1/20 | G3→G4 | ||
| 1/20 | A3→A4 | ||
| 1/20 | T6→T5 | ||
| 2/20 | T6→T7 | ||
| Base substitution | 10/20 (50) | ||
| 2/20 | C→A | ||
| 5/20 | G→T | ||
| 1/20 | T→G | ||
| 2/20 | C→G | ||
| Complex events | 1/20 (5) | ||
| 1/20 | TATgGGTTcTTT-GG→TATtGGTTtTTTtGG | ||
| pol30-104 | |||
| Gross deletions | 0/22 (0) | ||
| Gross insertions | 0/22 (0) | ||
| Frameshifts | 16/22 (73) | ||
| 1/22 | G1→G0 | ||
| 1/22 | G1→G2 | ||
| 1/22 | A6→A5 | ||
| 1/22 | C2→C1 | ||
| 4/22 | T4→T3 | ||
| 7/22 | T6→T7 | ||
| 1/22 | T1→T0 | ||
| Base substitution | 6/22 (27) | ||
| 2/22 | G→T | ||
| 2/22 | G→A | ||
| 1/22 | C→G | ||
| 1/22 | C→A | ||
| Complex events | 0/22 (0) | ||
| pol30-105 | |||
| Gross deletions | 1/28 (4) | 36 bp7 | |
| Gross insertions | 0/28 (0) | ||
| Frameshifts | 5/28 (18) | ||
| 3/28 | T7→T6 | ||
| 1/29 | T6→T5 | ||
| 2/28 | T2→T1 | ||
| 1/28 | A2→A1 | ||
| Base substitution | 21/28 (74) | ||
| 4/28 | G→A | ||
| 2/28 | G→T | ||
| 6/28 | C→A | ||
| 1/28 | C→T | ||
| 1/29 | C→G | ||
| 2/28 | T→G | ||
| 2/28 | T→C | ||
| 3/28 | T→A | ||
| Complex events | 1/28 (4) | ||
| pol30-108 | |||
| Gross deletions | 0/24 (0) | ||
| Gross insertions | 0/24 (0) | ||
| Frameshifts | 10/24 (42) | ||
| 1/24 | G3→G2 | ||
| 1/24 | G2→G1 | ||
| 1/24 | G1→G0 | ||
| 1/24 | A3→A2 | ||
| 1/24 | A5→A6 | ||
| 1/24 | T6→T5 | ||
| 4/24 | T6→T7 | ||
| Base substitution | 14/24 (58) | ||
| 3/24 | G→T | ||
| 4/24 | G→A | ||
| 1/24 | G→C | ||
| 2/24 | C→T | ||
| 4/24 | C→A | ||
| Complex events | 0/24 (0) | ||
| pol30-126 | |||
| Gross deletions | 2/22 (9) | 4 bp8 | |
| 10 bp9 | |||
| Gross insertions | 5/22 (23) | 38 bp insertion10 | |
| 13 bp insertion11 | |||
| 23 bp insertion12 | |||
| 27 bp insertion13 | |||
| 17 bp insertion14 | |||
| Frameshifts | 5/22 (23) | ||
| 2/22 | G1→G0 | ||
| 2/22 | T7→T8 | ||
| 1/22 | C2→C1 | ||
| Base substitution | 10/22 (45) | ||
| 5/22 | C→A | ||
| 1/22 | G→A | ||
| 1/22 | G→T | ||
| 2/22 | C→G | ||
| 1/22 | T→C | ||
| Complex events | 0/22 (0) | ||
| pol30-114 | |||
| Gross deletions | 1/28 (4) | 27 bp15 | |
| Gross insertions | 1/28 (4) | 34 bp16 | |
| Frameshifts | 4/28 (14) | ||
| 2/28 | C2→C1 | ||
| 1/28 | C1→C0 | ||
| 1/28 | G1→G0 | ||
| Base substitution | 19/28 (68) | ||
| 1/28 | C→T | ||
| 6/28 | C→A | ||
| 3/28 | C→G | ||
| 5/28 | G→A | ||
| 3/28 | G→C | ||
| 1/28 | G→T | ||
| Complex events | 3/28 (10) | ||
| 1/28 | GTTTTcTCACAA→GTTTTtTtCACAA | ||
| 1/28 | ATATcATATTTATttatGGG→ATATTTATTTATGCG | ||
| 1/28 | AAGAGAGaGcTTGAG→AAGAGAGGTTGAG |
a
Superscript numbers correspond to those preceding the sequences in Table 4. Altered nucleotides are in lowercase; relevant microhomologies are in boldface.