TABLE 5.
Summary of CAN1 mutation spectra for pol30 mutantsa
| Genotype | Group(s) | Substitution (%) | Frameshifts (%) | Deletions (%) | Insertions (%) | Complex events (%) |
|---|---|---|---|---|---|---|
| Wild typeb | 65 (1) | 25 (1) | 0 | 0 | 10 | |
| msh2b | 15 (4) | 85 (60) | 0 | 0 | 0 | |
| pol30-41 | 68 (1) | 32 (1) | 0 | 0 | 0 | |
| pol30-45 | B | 88 (1) | 4 (0.3) | 4 | 4 | 0 |
| pol30-52 | A | 20 (5) | 80 (48) | 0 | 0 | 0 |
| pol30-100 | C | 90 (6) | 10 (2) | 0 | 0 | 0 |
| pol30-102 | 69 (2) | 31 (2) | 0 | 0 | 0 | |
| pol30-103 | B, C | 50 (4) | 25 (4) | 20 | 0 | 0 |
| pol30-104 | A | 27 (11) | 73 (76) | 0 | 0 | 0 |
| pol30-105 | B, C | 74 (2) | 18 (1) | 4 | 0 | 4 |
| pol30-108 | A, C | 58 (4) | 42 (8) | 0 | 0 | 0 |
| pol30-126 | A, B | 45 (4) | 23 (6) | 9 | 23 | 0 |
| pol30-114 | B, C | 68 (2) | 14 (1) | 4 | 4 | 10 |
Indicated in parentheses is the fold increase in the estimated rate of frameshift and base substitution accumulation in various mutants over the wild-type rate. The estimated rates were calculated by multiplying the proportion of frameshifts and base substitutions (Table 2) by the respective CAN1 mutation rates (Table 1). Group A mutants exhibit mutation rates and spectra reminiscent of mutants defective in MMR. Group B mutants accumulated either deletions or a combination of deletions and duplications in addition to base substitutions and frameshifts. Group C mutants exhibit mutation rates which were synergistic in the presence of a msh6 mutation. Many pol30 mutants harbor multiple mutagenic defects and were placed into more than one group.
From reference 47.