TABLE 6.
In vivo MMR assay
Substrate | Genotype | RDKY no. | No. of sectored coloniesa | Total no. of coloniesa | % Sectoringa |
---|---|---|---|---|---|
A/C | |||||
Wild type | 3023 | 57 | 1,783 | 3.2 | |
msh2 | 2706 | 843 | 1,400 | 60.2 | |
pol30-100 | 3553 | 116 | 2,723 | 4.3 | |
pol30-103 | 3555 | 71 | 2,192 | 3.2 | |
pol30-104 | 3556 | 439 | 1,025 | 42.8 | |
pol30-108 | 3559 | 83 | 293 | 28.3 | |
pol30-126 | 3560 | 401 | 2,513 | 15.9 | |
G/T | |||||
Wild type | 3023 | 85 | 3,142 | 2.7 | |
msh2 | 2706 | 943 | 1,455 | 64.8 | |
pol30-100 | 3553 | 99 | 2,255 | 4.4 | |
pol30-103 | 3555 | 66 | 1,801 | 3.7 | |
pol30-104 | 3556 | 238 | 704 | 33.8 | |
pol30-108 | 3559 | 249 | 1,140 | 21.8 | |
pol30-126 | 3560 | 183 | 1,701 | 10.7 |
Results of a typical experiment, each repeated at least twice.