TABLE 7.
Rates of heteroallelic recombination and chromosome loss in msh2, pol30-104, and pol30-104 msh2 mutants
| Strain | Ratea
|
|
|---|---|---|
| Heteroallelic recombination | Chromosome loss | |
| RKY3579 (wild type/wild type) | 3.6 × 10−5 (1) | 3.2 × 10−6 (1) |
| RKY3580 (msh2/msh2) | 8.0 × 10−5 (2) | 4.2 × 10−6 (1) |
| RKY3581 (pol30-104/pol30-104) | 1.8 × 10−3 (50) | 2.3 × 10−4 (72) |
| RKY3582 (pol30-104 msh2/pol30-104 msh2) | 0.6 × 10−3 (17) | 0.7 × 10−4 (22) |
a
Average of two more more experiments. Numbers in parentheses indicate fold induction relative to the wild-type rate. The Mann-Whitney test indicates that the recombination rate and the chromosomal loss rate in pol30-104/pol30-104 mutants were significantly higher than those of pol30-104 msh2/pol30-104 msh2 diploids (P < 0.05).