Fig. 2.
Glutamate induces ionotropic NMDA receptors at the mRNA and protein levels. a MCs (1 × 106/ml) were exposed to glutamate (10 or 100 µM) for either 1 h or 4 h, followed by the measurement of the mRNA encoding NMDAR1, NMDAR2A, and NMDAR2B by qPCR. b Immunofluorescence staining for NMDAR1, NMDAR2A, and NMDAR2B in the MCs incubated for 24 h with glutamate (100 µM). c Quantification of the immunofluorescence staining, presented as the means ± SEM (n = 3). Significance was calculated with an unpaired t-test with Welch’s correction. *p ≤ 0.05; **p ≤ 0.01. Scale bars: 25 μm