. 2021 Sep 3;18(10):2344–2357. doi: 10.1038/s41423-021-00760-2

Table 1.

Enzyme activities in 3HB biosynthesis pathway encoded in EcN strains

Enzymes	Activities (U/mg) for E. coli Nissle 1917 strains^a
Acetyl-CoA acetyltransferase	Control	PhaA	BktB	ThlA
Acetyl-CoA acetyltransferase	<0.1	12.7 ± 2.2	2.6 ± 0.4	5.1 ± 0.9
3HB-CoA dehydrogenase	Control	PhaB_Cn	PhaB_TD
3HB-CoA dehydrogenase	ND^b	2.2 ± 0.3	1.9 ± 0.2
Thioesterase	Control	TesB	Ptb-Buk	Pct	YciA
Thioesterase	1.8 ± 0.3	40.6 ± 5.7	10.4 ± 2.7	8.7 ± 1.2	2.8 ± 1.1

^aOne activity unit was defined as the conversion of 1 mole of substrate to product during 1 min at 37 °C. Data are the average values and the standard deviations are calculated from three independent cultures

^bND not detected