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. 2021 Sep 30;11:19459. doi: 10.1038/s41598-021-98869-x

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Cq values of twelve candidate reference genes in human cancer (red) and normal (blue) cell lines. Each dot represents the average Cq value of three biological replicates for each cell line. Candidate reference genes are arranged according to the comprehensive ranking in a decreasing order of expression stability. The boxes represent the data between the 25th and 75th percentile, while whiskers show the data range from the 10th to the 90th percentile. The minimum and maximum values are depicted as ‘x’. The average values are represented as squares and the median values are illustrated as lines. This graph was created with OriginPro 2018 (OriginLab Corp.).