Table 4.
Ranking of candidate reference genes.
| Comprehensive rank | Gene symbol | GeNorm | Comparative ΔCt | NormFinder | BestKeeper | Geometric mean | ||||
|---|---|---|---|---|---|---|---|---|---|---|
| Rank | M value | Rank | Mean StdDev | Rank | Stability value | Rank | Stability value | |||
| All cell lines | ||||||||||
| 1 | IPO8 | 1 | 0.381 | 3 | 0.555 | 1 | 0.205 | 3 | 0.336 | 1.73 |
| 2 | PUM1 | 2 | 0.384 | 4 | 0.568 | 4 | 0.222 | 1 | 0.326 | 2.38 |
| 3 | HNRNPL | 3 | 0.386 | 1 | 0.538 | 3 | 0.215 | 6 | 0.353 | 2.71 |
| 4 | SNW1 | 4 | 0.407 | 2 | 0.545 | 2 | 0.206 | 4 | 0.344 | 2.83 |
| 5 | CNOT4 | 5 | 0.428 | 5 | 0.597 | 5 | 0.266 | 5 | 0.345 | 5.00 |
| 6 | GAPDH | 10 | 0.547 | 7 | 0.652 | 6 | 0.268 | 2 | 0.328 | 5.38 |
| 7 | PCBP1 | 6 | 0.45 | 6 | 0.612 | 7 | 0.273 | 7 | 0.394 | 6.48 |
| 8 | TBP | 7 | 0.477 | 9 | 0.678 | 9 | 0.342 | 11 | 0.483 | 8.89 |
| 9 | PPIA | 8 | 0.504 | 8 | 0.657 | 8 | 0.308 | 10 | 0.471 | 8.46 |
| 10 | RPL30 | 9 | 0.523 | 10 | 0.701 | 11 | 0.366 | 8 | 0.406 | 9.43 |
| 11 | UBC | 11 | 0.587 | 11 | 0.777 | 10 | 0.362 | 9 | 0.457 | 10.22 |
| 12 | ACTB | 12 | 0.636 | 12 | 0.911 | 12 | 0.503 | 12 | 0.679 | 12.00 |
| Cancer cell lines | ||||||||||
| 1 | HNRNPL | 1 | 0.333 | 1 | 0.475 | 1 | 0.160 | 1 | 0.275 | 1.00 |
| 2 | IPO8 | 2 | 0.346 | 3 | 0.535 | 2 | 0.212 | 5 | 0.322 | 2.78 |
| 3 | PUM1 | 3 | 0.363 | 4 | 0.539 | 4 | 0.215 | 4 | 0.32 | 3.72 |
| 4 | SNW1 | 5 | 0.416 | 2 | 0.533 | 3 | 0.213 | 8 | 0.368 | 3.94 |
| 5 | CNOT4 | 4 | 0.393 | 5 | 0.564 | 6 | 0.257 | 6 | 0.332 | 5.18 |
| 6 | GAPDH | 10 | 0.523 | 7 | 0.616 | 5 | 0.245 | 3 | 0.31 | 5.69 |
| 7 | RPL30 | 7 | 0.467 | 9 | 0.627 | 11 | 0.321 | 2 | 0.308 | 6.10 |
| 8 | UBC | 6 | 0.437 | 6 | 0.599 | 7 | 0.260 | 7 | 0.355 | 6.48 |
| 9 | PCBP1 | 9 | 0.503 | 8 | 0.623 | 8 | 0.299 | 9 | 0.394 | 8.49 |
| 10 | TBP | 8 | 0.487 | 10 | 0.640 | 10 | 0.312 | 10 | 0.447 | 9.46 |
| 11 | PPIA | 11 | 0.539 | 11 | 0.644 | 9 | 0.308 | 11 | 0.458 | 10.46 |
| 12 | ACTB | 12 | 0.595 | 12 | 0.904 | 12 | 0.510 | 12 | 0.716 | 12.00 |
| Normal cell lines | ||||||||||
| 1 | IPO8 | 1 | 0.223 | 1 | 0.495 | 1 | 0.149 | 3 | 0.318 | 1.32 |
| 2 | SNW1 | 2 | 0.236 | 3 | 0.515 | 2 | 0.187 | 1 | 0.29 | 1.86 |
| 3 | PUM1 | 3 | 0.26 | 2 | 0.508 | 5 | 0.218 | 4 | 0.323 | 3.31 |
| 4 | CNOT4 | 9 | 0.433 | 5 | 0.552 | 3 | 0.203 | 2 | 0.301 | 4.05 |
| 5 | PCBP1 | 6 | 0.368 | 4 | 0.544 | 4 | 0.214 | 6 | 0.386 | 4.90 |
| 6 | PPIA | 5 | 0.349 | 7 | 0.570 | 6 | 0.282 | 7 | 0.422 | 6.19 |
| 7 | HNRNPL | 4 | 0.327 | 6 | 0.568 | 7 | 0.286 | 9 | 0.455 | 6.24 |
| 8 | GAPDH | 10 | 0.481 | 9 | 0.678 | 8 | 0.306 | 5 | 0.368 | 7.75 |
| 9 | RPL30 | 7 | 0.387 | 8 | 0.632 | 9 | 0.318 | 8 | 0.437 | 7.97 |
| 10 | TBP | 8 | 0.407 | 10 | 0.685 | 10 | 0.374 | 10 | 0.533 | 9.46 |
| 11 | ACTB | 11 | 0.554 | 11 | 0.884 | 11 | 0.464 | 11 | 0.553 | 11.00 |
| 12 | UBC | 12 | 0.622 | 12 | 0.967 | 12 | 0.554 | 12 | 0.617 | 12.00 |
The comprehensive rank is generated based on the geometric mean of ranks. The best combinations of reference genes as suggested by NormFinder are shown in bold.