TABLE 2.

Summary of potential metabolites in the spleen.

No.	Metabolites	Formula	Mass (m/z)	Retention time (min)	Fold changea		p value		VIP valueb
					Con vs. IMQ	IMQ vs. ESW	Con vs. IMQ	IMQ vs. ESW	Con vs. IMQ	IMQ vs. ESW
1	Ureac	CH4N2O	132.10	6.61	0.75	1.27	0.00	0.13	1.14	1.15
2	Glycinec	C2H5NO2	174.20	6.72	0.85	0.76	0.02	0.09	2.24	3.65
3	2-Butenedioic acidc	C4H4O4	204.20	7.91	1.14	1.00	0.03	1.00	2.57	0.02
4	L-Threoninec	C4H9NO3	291.28	8.23	0.87	1.03	0.04	0.76	1.01	0.24
5	Fructosec	C6H12O6	326.20	11.86	3.07	0.43	0.03	0.17	1.49	1.32
6	Heptanoic acidc	C7H14O2	73.12	14.44	1.27	0.92	0.01	0.38	1.29	0.58
7	L-Ascorbic acidd	C6H8O6	72.20	16.24	0.23	2.38	0.00	0.02	1.46	1.56
8	Quinolinec	C9H7N	305.25	18.29	1.53	0.94	0.00	0.30	5.26	1.47
9	D-Glucosed	C6H12O6	204.20	16.48	2.25	2.38	0.02	0.02	2.93	1.56
10	L-Prolined	C5H9NO2	142.20	7.16	0.87	0.80	0.04	0.01	1.99	2.49
11	Pyrimidined	C4H4N2	241.20	7.66	0.69	1.85	0.01	0.00	1.48	4.07
12	Phosphoric acidd	H3PO4	357.2	13.14	0.34	1.69	0.00	0.03	3.10	2.73
13	L-Valinee	C5H11NO2	144.20	6.05	1.06	0.86	0.45	0.05	0.83	2.59
14	Malic acide	C4H6O5	232.20	9.95	0.92	1.15	0.07	0.01	2.56	4.82
15	L-Phenylalaninee	C9H11NO2	192.20	11.33	1.36	0.67	0.17	0.03	1.03	1.85
16	d-Mannosee	C6H12O6	179.20	15.12	1.02	0.78	0.82	0.00	0.23	3.22
17	Z,Z-9,12-Octadecadienoic acide	C18H32O2	253.20	20.35	3.71	0.23	0.17	0.03	0.76	1.34

^aFold change with a value >1 indicated a relatively higher intensity, whereas a value <1 indicated a relatively lower intensity.

^bVIP value was obtained from the OPLS-DA model.

^cVariables were obtained by comparing Con vs. IMQ (VIP >1 and p < 0.05).

^dVariables were obtained by comparing IMQ vs. Con and ESW (VIP >1 and p < 0.05).

^eVariables were obtained by comparing IMQ vs. ESW (VIP >1 and p < 0.05).