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. 2021 Oct 1;8:219. doi: 10.1038/s41438-021-00654-4

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — A Yeast two-hybrid (Y2H) analysis showing that both MdPR10-1 and MdPR10-2 interact with MdRNL2 and MdRNL6. Coexpression of SV40-AD/BD-P53 was used as a positive control. As negative controls, MdPR10-1-AD/BK, MdPR10-2-AD/BK, AD/MdPR10-1-BK, AD/MdPR10-2-BK, and AD/BD were coexpressed. B β-Galactosidase activity in Y2H assays. The error bars show the standard error of the mean (SEM) values (n = 3 biological replicates). **P < 0.01 (Student’s t test). C BiFC assay of the interactions between MdPR10-1 and MdRNL2–MdRNL6 and between MdPR10-2 and MdRNL2–MdRNL6. MdRNL2-YFPc/MdPR10-1-YFPn, MdRNL2-YFPc/MdPR10-2-YFPn, MdPR10-1-YFPc/MdRNL6-YFPn, and MdPR10-2-YFPc/MdRNL6-YFPn were transiently expressed in N. benthamiana epidermal cells. 1300-YFPc/MdPR10-1-YFPn, 1300-YFPc/MdPR10-2-YFPn, MdPR10-1-YFPc/1300-YFPn, and MdPR10-2-YFPc/1300-YFPn were used as negative controls. Scale bars = 50 μm