Fig. 3.

Effects of PCBP1 deficiency on the activation of downstream components of cGAS. a, b Effects of PCBP1 deficiency on the dsDNA-induced synthesis of cGAMP in the MLFs. PCBP1-deficient and control MLFs (1 × 10⁷) were mock-transfected or transfected with HT-DNA (5 μg) for 6 h. Cell extracts containing cGAMP were delivered to digitonin-permeabilized MLFs and incubated for 4 h before qPCR (a), or cGAMP in the cell extracts was measured by ELISA (b). The graph shows the means ± SEM, n = 3. **P < 0.01 (unpaired t-test). c Effects of PCBP1 deficiency on the HSV-1-induced phosphorylation of downstream components in the MLFs. PCBP1-deficient and control MLFs (1 × 10⁶) were left untreated or infected with HSV-1 (MOI = 1) for the indicated times before the immunoblot analysis was performed with the indicated antibodies. d Deficiency of PCBP1 has no effects on IFN-β-induced phosphorylation of STAT1 in the MLFs. PCBP1-deficient and control MLFs (1 × 10⁶) were untreated or treated with IFN-β (100 ng/mL) for the indicated times before the immunoblot analysis was performed