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The strategy for the creation of cellular COG-CDG models. A conceptual design of the COG4-SWS and COG4-CDGIIj cellular models. In the first step CRISPR/Cas9 protocol was used to make RPE1 and HEK293T COG4 KO cells lines. On the second step, lentiviral transduction and G418 selection was utilized to create populations of isogenic cells expressing GFP or 3myc-tagged COG4 variants under endogenous COG4 promoter.
