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. 2021 Sep 17;12:733048. doi: 10.3389/fgene.2021.733048

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Copyright © 2021 Sumya, Pokrovskaya and Lupashin.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Expression of G516R and R729W mutant proteins rescues major cellular phenotypes associated with COG4 deficiency. (A) WB to detect COG4, myc, B4GalT1, Lamp2, Tmem165, and actin in total cellular lysates prepared from RPE1 wild type, COG4 KO, rescued COG4-WT-3myc, COG4-G516-3myc, and COG4-R729W-3myc cells. Actin has been used as a loading control. (B) Airyscan superresolution IF analysis of RPE1 cells stained for myc (red), Giantin (green), B4GalT1 (blue). The red, green and infrared (blue) channels are presented in inverted black and white mode, whereas the merged view is shown in RGB mode. Scale bars, 20 µm.