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. 2021 Sep 17;12:733048. doi: 10.3389/fgene.2021.733048

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Copyright © 2021 Sumya, Pokrovskaya and Lupashin.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Expression of COG4 mutants does not alter Golgi morphology. (A) Airyscan superresolution IF analysis of RPE1 COG4 wild type, G516R and R729W rescued cells stained for (A) GM130 (green) and TGN46 (red) and (B) GM130 (green) and ERGIC53 (red). Scale bars, 20 µm. Colocalization of TGN46 vs GM130 and ERGIC53 vs GM130 has been performed using Pearson’s correlation coefficient (Right panel for both (A) and (B), n = 20. Statistical significance was calculated by GraphPad Prism 8 using one-way ANOVA. p > 0.05, non-significant (ns). Error bar represents mean ± SD.