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. 2021 Sep 17;12:733048. doi: 10.3389/fgene.2021.733048

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Copyright © 2021 Sumya, Pokrovskaya and Lupashin.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Expression of G516R and R729W mutants do not affect the localization of v-SNARE GS15. (A) RPE1 rescued COG4-WT-3myc, COG4-G516R-3myc, and COG4-R729-3myc cells were stained with GM130 (green) and GS15 (red). Scale bar 20um. (B) Colocalization of GS15 with GM130 has been analyzed using Pearson’s correlation coefficient. Quantification of colocalization in 20 cells analyzed. Statistical significance was calculated by GraphPad Prism 8 using one-way ANOVA. p > 0.05, nonsignificant (ns). Error bar represents mean ± SD.