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. 2021 Sep 17;9:684857. doi: 10.3389/fcell.2021.684857

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Copyright © 2021 Liu, Dai, Wang, Feng and Xiao.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Tunicamycin concentration and treatment time screening by MTT. After the HSCs were treated by TM with different concentrations and treatment time, the OD value of each treatment was assayed, and the proliferation rate (PR) of HSCs was calculated. (A) HSCs were treated with various concentrations of TM for 24 h. (B) HSCs were incubated in the presence of 2 μg/ml TM for the indicated time periods.