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. 2021 Sep 17;12:714138. doi: 10.3389/fimmu.2021.714138

Figure 4.

Figure 4

Infliximab (IFX) induces annexin A1 (AnxA1) expression and secretion by infiltrated leukocytes during inflammation. (A–E) AnxA1 expression in the epithelial barrier from wild-type (WT) mice colon. AnxA1 concentrated on the wound bed (white arrows). (F–I) Immunostaining for AnxA1 in submucosal leukocytes. Tissue leukocytes positive (black arrowheads) or negative (white arrowheads) for AnxA1 staining. (J) Reaction control. Counterstaining, hematoxylin; embedding, paraffin; sections, 3 μm. Bars, 20 μm (A–E) and 100 μm (F–I). (K) AnxA1 secreted by lamina propria leukocytes cultured ex vivo. (L) AnxA1 secreted by colon explants in culture. (M) Moderate negative correlation between AnxA1 secreted into the tissue and histological grading. N.D., non-detected. *p < 0.05, **p < 0.01. n = 3–4 mice/group (K); n = 4–6 mice/group (L). Results are expressed as the mean ± SEM.