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. 2021 Sep 17;9:687258. doi: 10.3389/fcell.2021.687258

FIGURE 5.

FIGURE 5

H-Exo and L-Exo promoted the osteogenic differentiation of TCs through PINK1/Parkin-mediated mitophagy. (A) Osteogenic inducing fluid containing 20 μg/ml exosomes was used, and the expression levels of the osteogenic differentiation-related genes in the TCs without transfection or transfected with scrambled siRNA or PINK1 siRNA and with L-Exo stimulation were determined by RT-qPCR after 7 days of osteogenic induction (n = 3). (B) Osteogenic inducing fluid containing 20 μg/ml exosomes was used, and the expression levels of osteogenic differentiation-related genes in the TCs without transfection or transfected with scrambled siRNA or PINK1 siRNA and with H-Exo stimulation were determined by RT-qPCR after 7 days of osteogenic induction (n = 3). (C) Osteogenic inducing fluid containing 20 μg/ml exosomes was used, and mineralization nodule formation in the TCs without transfection or transfected with scrambled siRNA or PINK1 siRNA and with L-Exo stimulation was tested by Alizarin red staining after 28 days of osteogenic induction (n = 3). (D) Osteogenic inducing fluid containing 20 μg/ml exosomes was used, and mineralization nodule formation in the TCs without transfection or transfected with scrambled siRNA or PINK1 siRNA and with H-Exo stimulation was tested by Alizarin red staining after 28 days of osteogenic induction (n = 3). L-Exo, exosomes secreted by L-SCCs; L-Exo + scramble, TCs transfected scrambled siRNA with L-Exo stimulation; L-Exo + siPINK1, TCs transfected PINK1 siRNA with L-Exo stimulation; H-Exo, exosomes secreted by H-SCCs; H-Exo + scramble, TCs transfected scrambled siRNA with H-Exo stimulation; H-Exo + siPINK1, TCs transfected PINK1 siRNA with H-Exo stimulation. *P < 0.05; **P < 0.01; ***P < 0.001.