TABLE 2.

Differences in plate-level amplification efficiency as a function of amplicon target and calculation approach.

Approach	T plate efficiency	S plate efficiency	p-value (between amplicons)
(1) Rotor-gene Q standard-curve	1.89 (0.03)	2.12 (0.04)	1.40E−12
(2) LinRegPCR standard-curve	1.83 (0.04)	2.10 (0.05)	4.55E−11
(3) LinRegPCR reconstructed during exponential phase	1.86 (0.05)	1.92 (0.05)	2.57E−08
p-value between approaches (1 vs. 2)	7.51E−08	2.98E−04
p-value between approaches (2 vs. 3)	0.129	1.02E−08
p-value between approaches (1 vs. 3)	0.048	1.34E−09

Efficiency values are mean (standard deviation) across 17 T plates or 17 S plates. Reported p-values are results of paired sample t-tests across 17 pairs of quantitative-PCR (qPCR) plates.