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CM-AcMSC inhibited the expression and production of cytokines in colon tissues, n = 6. (A) The mRNA levels of cytokines such as TNF-α, IL-1β, IL-6, and IL-10 were determined by quantitative reverse-transcription PCR, normalized by β-actin. (B) The protein levels of TNF-α,IL-1β, IL-6, and IL-10 were detected by ELISA. Data are presented as means ± SEM. † p < 0.05, †† p < 0.01 vs CTR; # p < 0.05, ## p < 0.01 vs DSS; * p < 0.05, ** p < 0.01.
