Figure 3.

Erianin induces pro-survival autophagy in LNCaP cells. (A) LNCaP cells were treated with erianin at indicated concentrations for 24 h in the presence or absence of 100 nM bafilomycin A1 (BafA1, an autophagy inhibitor). Levels of p62, LC3, cleaved caspase-3 and cleaved PARP were examined by Western blotting. FL-, full length; c-, cleaved; casp3, caspase 3. (B) LNCaP cells were transfected with GFP-tagged LC3 for 48 h. Then cells were co-treated with 100 nM BafA1 and 50 nM erianin for 24 h. Confocal images were captured. Bar = 10 µm. (C) Cells were treated with erianin at indicated concentrations in the presence or absence of 100 nM bafilomycin A1 for 24 h. Cell viability was determined by MTS colorimetric assay. Data are expressed as mean ± SD. n=4. *p < 0.05, -BafA1 versus +BafA1.