FIGURE 2.

Interleukin-13 alleviates cardiomyocyte apoptosis and ROS production in vitro. (A) PI and Annexin V dye was used to observe the level of apoptosis. (B) PI positive cells were counted by flow cytometry and (C) calculated. Annexin V positive cells (D) and the apoptosis ratio (E) were counted by flow cytometry and calculated. (F) DCFH-DA staining was applied to detect the level of ROS and (G) measured with image J. Data are shown as the mean ± SEM (n = 3). *P < 0.05; **P < 0.01; ***P < 0.001.