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Representative chromatogram of ELA-32(1-16) (A) and ELA-16 (B) produced from the hydrolysis of ELA-32. ELA-32(1-16) and ELA-16 were generated from the cleavage between Asn16 and Cys16. ELA-32(1-16), retention time 32.65 min; ELA-16 was only present in the oxidised form (methionine oxidation); retention time 40.80 min. The red line indicates the cleavage point.
