Table 1.
Primer used for SYBR Green I qRT-PCR validation.
| Gene Symbol | Primer Sequence 5’-3’ (Forward) | Primer Sequence 5’-3’ (Reverse) |
|---|---|---|
| homo-GAPDH | GGGAAGCTTGTCATCATCAATGG | CATCGCCCCACTTGATTTTG |
| homo-IL-1β | GTACCTGAGCTCGCCAGTG | TGTTTAGGGCCATCAGCTT |
| homo-IL-6 | GACAGCCACTCACCTCTTCA | TTCACCAGGCAAGTCTCCTC |
| homo-TNF-α | CCGAGTCTGGGCAGGTCTA | CGTTTGGGAAGGTTGGATG |
| homo-ZO-1 | CGGGACTGTTGGTATTGGCTAGA | GGCCAGGGCCATAGTAAAGTTTG |
| homo-Claudin-1 | GCGCGATATTTCTTCTTGCAGG | TTCGTACCTGGCATTGACTGG |
| homo-Muc-2 | AACGGCCTGCAGAGCTATTC | ATCTTCTGCATGTTCCCAAACTC |
| homo-NRF2 | CGACGGAAAGAGTATGAG | TGGGAGTAGTTGGCAGAT |
| homo-SOD1 | GACAGCCACTCACCTCTTCA | CTTCATTTCCACCTTTGC |
| homo-GPx1 | AAGGTACTACTTATCGAGA ATGTG | GTCAGGCTCGATGTCAATGGTCTG |
| homo-NOX2 homo-Keap1 | AATCCCTGCTCCCACTAACAATGCAGCCAGATCCA | TTTCAAGATGCGTGGAAACTACGCAGAACTGTACCTGTTGA |
| homo-Keap1 | ATGCAGCCAGATCCA | GCAGAACTGTACCTGTTGA |
| mus-GAPDH | ACCCCAGCAAGGACACTGAGCAAG | GGCCCCTCCTGTTATTATGGGGGT |
| mus-SOD1 | TTCGAGCAGAAGGCAAGCGGTGAAA | AATCCCAATCACACCACAAGCCAA |
| mus-SOD2 | CAGACCTGCCTTACGACTATGG | CTCGGTGGCGTTGAGATTGTT |
| mus-IL-6 | AGACAAAGCCAGAGTCCTTCAGAGA | GCCACTCCTTCTGTGACTCCAGC |
| mus-TNF-α | AGCAGGCCATCACCACCAAGA | GTGCGTCACATCCTTGAAGTCAT |
| mus-NOX2 | TGTTTTCATTTCCTCATCAGAAG | CCAACCACACCAGAATGACA |
| mus-Nrf2 | CGAGATATACGCAGGAGAGGTAAGA | GCTCGACAATGTTCTCCAGCTT |
| Homo means Homo sapiens and mus means Mus musculus | ||