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. 2021 Sep 17;9:719636. doi: 10.3389/fcell.2021.719636

FIGURE 5.

FIGURE 5

Effect of potential upstream regulators on basal and BS derivative-stimulated neurite outgrowth. (A) Representative images of eGFP-NPCs treated with vehicle (DMSO), 10 μM Y27632 (Y27, ROCK1 inhibitor), 10 μM SP600125 (SP600, JNK inhibitor), or with the combination of Y27 and SP600. Images were acquired 4 h after treatment. Scale bars indicate 10 μm. (B) The neurite outgrowth rates were determined in NPCs treated with NBS, AmBS, Y27, SP600, or combinations of those inhibitors. Vehicle control (DMSO) was used as a reference point. The ROCK1 inhibitor stimulated neurite outgrowth to similar extent to that seen with NBS and AmBS. In contrast, JNK inhibition had no effect on neurite outgrowth. When NBS was co-administered with Y27632 or SP600125, neither additivity nor inhibition was seen. Similar results were obtained with AmBS. Similar to NBS and AmBS, SP600125 did not alter Y27632-stimulated neurite outgrowth. All panels depict the means ± SEM from 3 to 5 independent experiments. Asterisks indicate significant differences as compared to the DMSO control, whereas section signs mark significant differences as compared to samples, which are not treated with NBS or AmBS in the same group (p < 0.05).