Fig. 5.

Effect of reduced expression of 2-OGDH E₂ subunit on metabolite levels of the main nitrogen-containing compounds in fully expanded leaves of Arabidopsis. (A) Chlorophyll a; (B) chlorophyll b; (C) chlorophyll a/b; (D) total amino acids; (E) total soluble protein; and (F) nitrate. Metabolite levels were determined in 4-week-old fully expanded leaves harvested in the middle of the light period. Values are presented as means ± SE of five individual plants per line. Different letters indicate a significant difference at P < 0.05 using one-way ANOVA followed by post hoc Tukey's test, alpha = 0.05.