Table I.
siRNA sequences.
| siRNA | Sequence, 5′-3′ |
|---|---|
| siATF4-1 | |
| Forward | GUCCUCCACUCCAGAUCAUTT |
| Reverse | AUGAUCUGGAGUGGAGGACTT |
| siATF4-2 | |
| Forward | UGGAUAUCACUGAAGGAGATT |
| Reverse | UCUCCUUCAGUGAUAUCCATT |
| siXBP1-1 | |
| Forward | GCAAGUGGUAGAUUUAGAATT |
| Reverse | UUCUAAAUCUACCACUUGCTT |
| siXBP1-2 | |
| Forward | CCUAAAGUUCUGCUUCUGUTT |
| Reverse | ACAGAAGCAGAACUUUAGGTT |
| siATF6-1 | |
| Forward | GAAAUGUCGGUUCAGAUAUTT |
| Reverse | AUAUCUGAACCGACAUUUCTT |
| siATF6-2 | |
| Forward | GAGCCACUGAAGGAAGAUATT |
| Reverse | UAUCUUCCUUCAGUGGCUCTT |
| siCHAC1-1 | |
| Forward | GACGCUCCUUGAAGAUCAUTT |
| Reverse | AUGAUCUUCAAGGAGCGUCTT |
| siCHAC1-2 | |
| Forward | GCCACAACCUUGAAUACUUTT |
| Reverse | AAGUAUUCAAGGUUGUGGCTT |
| si*CHAC1 | |
| Forward | UGGGAAGCUCAUCACUACATT |
| Reverse | UGUAGUGAUGAGCUUCCCATT |
| Negative control | |
| Forward | UUCUCCGAACGUGUCACGUTT |
| Reverse | ACGUGACACGUUCGGAGAATT |
siRNA, small interfering RNA; XBP1, X-box binding protein 1; ATF4, activating transcription factor 4; ATF6, activating transcription factor 6; CHAC1, ChaC Glutathione Specific γ-glutamylcyclotransferase 1.