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. 2021 Sep 20;46(5):239. doi: 10.3892/or.2021.8190

Figure 4.

Figure 4.

circ-FOXO3 increases LATS1 expression via sponging miR-543. (A) Luciferase assay was conducted to verify whether miR-543 directly binds to LATS1 in HT29 and HCT116 cells. (B) Transfection efficiency of miR-543 mimic and inhibitor in HT29 and HCT116 cells was determined using cell fluorescence. Magnification, ×400; Scale bar, 50 µm. (C) mRNA and (D) protein expression levels of LATS1 in cells transfected with miR-543 mimic or inhibitor was determined using RT-qPCR and western blotting, respectively. (E) Expression of circ-FOXO3 in HT29 and HCT116 cells transfected with miR-543 mimic or inhibitor was determined using RT-qPCR. (F) mRNA and protein expression levels of LATS1 in cells transfected with pcDNA circ-FOXO3 or co-transfected with pcDNA circ-FOXO3 and miR-543 mimic in HT29 and HCT116. (G) RT-qPCR assay examined the mRNA expression of LATS1 in tumor tissues and adjacent healthy tissues of 70 patients with CRC. (H) Pearson correlation analysis determined the correlation between the expression levels of LATS1 and circ-FOXO3. All experiments were performed three times. **P<0.01, ***P<0.001, ****P<0.0001 vs. miR-NC group. circ-FOXO3, circular RNA FOXO3; RT-qPCR, reverse transcription-quantitative PCR; miR, microRNA; NC, negative control; WT, wild-type; MUT, mutant; LATS1, Large tumor suppressor kinase 1.