Skip to main content
. 2021 Sep 20;46(5):239. doi: 10.3892/or.2021.8190

Figure 5.

Figure 5.

miR-543 overexpression or LATS1 knockdown blocks circ-FOXO3-induced attenuated CRC aggressive features. (A) Cell Counting Kit-8 assay was conducted to determine the viability of HT29 and HCT116 cells after the transfection of pcDNA circ-FOXO3, miR-543 mimic or si-LATS1. (B) Colony formation assay determined the colony formation ability of cells after the transfection of pcDNA circ-FOXO3, miR-543 mimic or si-LATS1. Transwell migration assay was conducted to assess the (C) migratory and (D) invasive abilities of HT29 and HCT116 cells after the transfection of pcDNA circ-FOXO3, miR-543 mimic or si-LATS1. Magnification, ×100; Scale bar, 100 µm. (E) Western blotting was performed to detect the protein expression levels of E-cadherin and N-cadherin in cells after transfection of pcDNA circ-FOXO3, miR-543 mimic or si-LATS1. All experiments were performed three times. **P<0.01, ***P<0.001, ****P<0.0001 vs. Vector group; #P<0.05, ##P<0.01, ###P<0.0001 vs. circ-FOXO3 group. circ-FOXO3, circular RNA FOXO3; LATS1, Large tumor suppressor kinase 1; miR, microRNA; siRNA, small interfering RNA; OD, optical density.