Figure 1.

Supervised AI computer-vision algorithms are trained using manual ground truth to achieve accurate cell segmentations on a specific domain of image data (left). A trained algorithm can then be deployed to automatically extract quantitative features from microscopy data to answer clinic- and biology-related questions (right). CNN, convolutional neural network; GAN, generative adversarial networks. Fiji, NIH, Bethesda, MD; https://imagej.nih.gov/ij. Ilastik; http://ilastik.org. ImageJ, NIH; http://imagej.nih.gov.ij.