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. 2021 Sep 17;11:719542. doi: 10.3389/fcimb.2021.719542

Figure 2.

Figure 2

BefA protein reduced intestinal inflammation and improved intestinal permeability. (A) Western blot analysis of ZO-1 and Occludin expression in colon tissues (n = 6), β-actin was used as an internal control. The relative expressions of ZO-1 and Occludin were quantified by Image J. (B) Western blot analysis of TLR-4, MyD88, p-p65 and p65 expression in colon tissues (n = 6), β-actin was used as an internal control. The relative expressions of TLR-4, MyD88, p-p65 and p65 were quantified by Image J. (C) Pro-inflammatory cytokines IL-1β, IL-6 and TNF-α were detected in colon tissues at gene level by q-PCR (n = 6). Data are presented as means ± SD. One way repeated-measures ANOVA with Tukey’s test for multiple comparisons (A–C, respectively); *p < 0.05, **p < 0.01.