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. 2021 Mar 4;104(6):1337–1346. doi: 10.1093/biolre/ioab036

Figure 3.

Figure 3

Neurotensin (NTS) mRNA expression in human GLC in vitro. Human GLCs were collected at the day of IVF retrieval (day 0) and NTS expression was examined across the 7 days of culture (A). After 6–7 days of culture, GLCs were serum starved for 1 h and then treated with vehicle control (−) or hCG (+) for 24 h (B). “Coll” represents samples at the time of IVF collection. A total of 0 h represents samples after 6–7 days in culture at the initiation of hCG treatment. Results are the means ± standard error of mean of n = 3. A two-way analysis of variance was used to test differences with or without hCG treatment across the time points. The Tukey post hoc test was performed and bars that do not share a letter designation are significantly different (p < 0.05).