Figure 3 .

DIP2B and SFMBT1 knockdown affects cell migration and invasion in vitro by transwell assays. (A) Migration and invasion assays were performed in RKO and SW620 cell lines transfected with U6 (control) and shRNA lentiviruses. After 24 h, migratory or invasive cells were stained with DAPI and imaged with the excitation wavelengths of 360–400 nm by using a Leica DM4000 microscope (magnification ×20). The independent experiments were performed in triplicate. (B) Quantification of DAPI-positive cells on the membrane. Images were taken for every membrane. The data are the mean ± SEM; ^*P < 0.05, ^**P < 0.01, ^***P < 0.001.