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. 2021 Sep 29;30:09636897211045319. doi: 10.1177/09636897211045319

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© The Author(s) 2021

This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).

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Figure 4. — Chondral regeneration effects of PDB on nicotine-treated 3D neo-cartilage IVD model. We conducted (A) Hematoxylin & eosin (H&E) staining (a-c, upper panel), immunohistochemistry staining of Col II (IHC Col II) (d-f, middle panel) and alcian blue staining (g-i, lower panel) to ascertain the morphologic alterations, collagen and proteoglycan expressions, respectively in control, nicotine-treated and PDB-treated nicotine-IVD group. All the images were captured at 20X magnification (Scale bar: 200 µm). Additionally, these staining signals of (B) Col II and (C) alcian blue were relatively quantified. The results are presented as mean ± S.D. (n = 3; *** P < 0.001).