TABLE 5.
Effect of Gal1p on the two-hybrid interaction of BDG80p and G4ADVP16p
| Proteins | Relevant genotype of host strain | β-Galactosidase activitya in:
|
Ratiob | |
|---|---|---|---|---|
| Gly-Lac | Gal-Gly-Lac | |||
| BDG80 | gal4Δ gal80Δ gal3Δ GAL1 | 5.09 | 4.00 | 1.3 |
| BDG80 + G4ADVP16 | gal4Δ gal80Δ gal3Δ GAL1 | 427.27 | 218.55 | 2.0 |
a
β-Galactosidase activity in yeast protein extracts is expressed in arbitrary units (1 unit = 1,000OD420 per min per mg of protein). The values are averages of four independent transformants. Standard errors were 3 to 5%. The yeast host strain was Sc798 (gal4Δ gal80Δ gal3Δ).
b
Ratio of β-galactosidase activity in Gly-Lac to that in Gal-Gly-Lac.