TABLE 7.
Compared to wild-type Gal4p, mutant Gal4ps lacking the middle region show reduced two-hybrid interaction with VP16Gal80p in the presence of galactose
| Plasmids | Proteins | β-Galactosidase activitya in:
|
|
|---|---|---|---|
| Gly-Lac | Gal | ||
| pLEUG4 + pAKS87 | Wild-type Gal4 + VP16Gal80 | 4,167 | 6,157 |
| pSB32-miniGal4-2 + pAKS87 | Mini-Gal4-2 + VP16Gal80 | 5,830 | 3,495 |
| pSB32-Gal4mCla + pAKS87 | Gal4mCla + VP16Gal80 | 5,925 | 3,840 |
a
β-Galactosidase activity in yeast protein extracts is expressed in arbitrary units (1 unit = 1,000OD420 per min per mg of protein). The values are averages of four independent transformants. Standard errors were 5 to 10%. The yeast host strain was Y187 (gal4Δ gal80Δ URA3::GAL1-lacZ).