FIGURE 5.

SEV‐derived miR‐574‐5p decreases mPGES‐1 and PGE₂ via TLR7/8 activation in A549 cells. (a, b) Western blot analysis of A549 and 2106T cells treated with 2 μg/ml sEV, 100 ng/ml R848 (TLR7/8 ligand) or 200 mM ODN 2088 Control (ODN 2087) (TLR7/8 antagonist). Cells were treated for 24 h. MPGES‐1 levels were normalized to GAPDH and folded to untreated cell samples (A549: N = 3‐5, 2106T: N = 5‐6). (c) PGE₂‐levels of A549 cells. 24 h after stimulation, cells were treated with 10 μM arachidonic acid (AA) for 15 min. PGE₂‐levels were measured via an enzyme‐linked immunosorbent assay (Cayman Chemicals, N = 4). Results are shown as mean +SEM, unpaired t‐test to other samples, *P≤0.05; **P≤0.01; ***P≤0.001; ****P≤0.0001. Unpaired t‐test to untreated control § P≤0.05; §§§ P≤0.001; §§§§ P≤0.0001