Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Jul 23;106(10):2641–2653. doi: 10.3324/haematol.2019.226241

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright© 2021 Ferrata Storti Foundation

This article is distributed under the terms of the Creative Commons Attribution Noncommercial License (by-nc 4.0) which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and source are credited.

PMC Copyright notice

Figure 6. — Effect of vitronectin and plasminogen activator inhibitor-1 heteromers on clustering of neutrophil b2 integrins. (A) Representative images of LFA-1/CD11a re-distribution and clustering in single neutrophils perfused over surfaces coated with E-selectin/CD62E, ICAM-1/CD54, and CXCL1 or CXCL1/VN-PAI-1 at indicated shear stress levels. LFA-1/CD11a expression is shown in pseudocolors as indicated by the lookup table (scale bar: 10 mm). (B) Scatter plot analysis of co-localized actin sum intensities versus co-localized LFA-1/CD11a sum intensities of single cells at indicated shear stress levels. (C) Quantification of actin co-localization and LFA-1/CD11a co-localization per clustered area at indicated shear stress levels (E-Selectin/CD62E + ICAM-1/CD54 coating: light green/yellow; E-selectin/CD62E + ICAM-1/CD54 + CXCL1 coating: dark green/brown; E-selectin/CD62E + ICAM-1/CD54 + CXCL1 + VN-PAI-1: grey; mean±standard deviation for n=3 independent experiments, n=6 flow chambers/coating, n=40-56 single cells/coating; *P<0.05 vs. E-selectin/CD62E + ICAM-1/CD54 coating).