Figure 1.

Storage time-dependent changes in biochemical, morphological and functional red blood cell parameters. Biochemical parameters: (A) cholesterol, (B) triglycerides, (C) lactate dehydrogenase (LDH), (D) free iron of human packed red blood cells (pRBC), (E) glucose, (F) lactate; chosen protein expression: (G) CD47 and (H) phosphatidylserine (PS); (I) pRBC deformability; the blood count including: (J) mean corpuscular volume (MCV), (K) hematocrit (HCT), (L) mean corpuscular hemoglobin concentration (MCHC). The blood was withdrawn from men (n=12) and women (n=12). Results (A to F) were obtained from pRBC supernatant with ABX Pentra 400 (Horiba Medical, Japan) are given as mean ± standard deviation (SD), data distribution is presented as box plots (mean value, mean ± SD, min-max whiskers). Results (G to L) presented as box plots (median, Q1, Q3, interquartile range, min-max whiskers). Q1, Q3 indicate 25th and 75th percentiles, respectively. Results (G and H) were obtained using a mixture of mouse anti-human antibodies carrying fluorescent markers: CD45 – APC-Cy7 (1:200, BD cat. no. 348815), CD71 – APC (1:200, BD cat. no. 551374), CD47 – PE (1:200, BD cat. no. 556046) and annexin V – FITC (1:200, BD cat. no. 556547). Cellular analysis was performed with LSRII flow cytometer (BD Biosciences). RBC deformability was studied at 37°C as elongation index at shear rates of 20 Pa with the use of ektacytometer RheoScanAnD 300 (RheoMeditech, Seoul, Korea) while parameters (J and L) with hematology analyzer Abc Vet (Horiba Medical, France). Data (A to L) normality was assessed using Shapiro-Wilk test. The significance of the differences between men and women in each week was evaluated by One-Way ANOVA with Tukey’s test. If data distribution was not normal, non-parametric Kruskal-Wallis test with the Dunn’s post hoc test if appropriate; *P<0.05, **P<0.01, ***P<0.001 ****P<0.0001 indicates significant difference between men and women in each week.