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. 2020 Aug 27;106(10):2598–2612. doi: 10.3324/haematol.2019.231456

Figure 1.

Figure 1.

The addition of CLL conditioned media, CLL sera or CLL cells to osteo-induced bone marrow stromal cells impairs their differentiation. The addition of CLL conditioned medium (cm), CLL sera (sr) or CLL cells, in contact (co) or in transwell (tw), to cultures of bone marrow stromal cells (BMSC), induced to differentiate in standard osteogenic medium, inhibited their complete differentiation to osteoblasts. RUNX2 and osteocalcin transcript levels were significantly decreased. In contrast, DKK-1 and osteopontin mRNA levels were increased when CLL-cm, CLL-sr or CLL cells were added to the cultures, as compared with levels when control BMSC were cultured with osteogenic medium only. Given the extremely high average and corresponding standard deviation values of DKK-1 mRNA levels, statistical significance was not achieved for CLL-cm and CLL-sr treatment. The mRNA expression of the same genes of interest was also evaluated in co-cultures of B cells purified from normal controls (B ctrl). n: number of cases examined for each experimental condition.