Figure 4.

Evaluation of the size of osteoclasts derived from monocytes cultured with MCSF and CLL-conditioned media in the presence or absence of RANKL, and of the mRNA expression of key molecules involved in this process. (A, B) TRAP⁺ cells, at least tri-nucleated, derived in the presence of MCSF+CLL-conditioned media (cm) (n=3; FA35, SD36, TC38) were smaller than osteoclasts classically derived with MCSF+RANKL or MCSF+RANKL+CLL-cm. (C) Size distribution of osteogenically induced monocytes with MCSF+RANKL or MCSF+CLL-cm. The curves depict the percentages of cells that fall within the limits of each class size (square pixels). (D) Cathepsin K, MMP9 and NFAT-C1 mRNA levels were higher in osteoclasts derived with CLL-cm. (E) The levels of cathepsin K mRNA expression were significantly higher in osteoclasts derived with CLL-cm in the presence of RANKL than without RANKL.