Table 2.
Reagents used for OMIP-0
| MARKER | ANTIBODY CLONE | FLUOROCHROME | PURPOSE |
|---|---|---|---|
|
| |||
| CD16 | 3G8 | BUV395 | NK, monocyte, ILC gating |
| Viability | NA | Fixable blue | Dead cell dump |
| CD3 | UCHT1 | BUV496 | T cells |
| CD19 | SJ25C1 | BUV563 | B cells |
| HLA-DR | G46–6 | BUV661 | Activation |
| CD27 | L128 | BUV737 | NK phenotyping |
| CD8 | SK1 | BUV805 | CD8+ T cells |
| TCRγδ | 11F2 | BV421 | Pan-γδ T cells |
| NKG2D | 1D11 | BV480 | NK phenotyping |
| CD56 | HCD56 | BV570 | NK phenotyping |
| Vδ2 TCR | B6 | BV605 | γδ T cell subset vδ2 |
| CD38 | HB-7 | BV650 | Activation |
| CD57 | HNK-1 | BV711 | NK phenotyping |
| CD4 | SK3 | BV750 | CD4+ T cells |
| TCRvα7.2 | 3C10 | BV786 | MAIT cells |
| FCεRIγ | Poly | FITC | NK phenotyping |
| Ki-67 | Ki67 | BB660 | Proliferation |
| CD14 | M®P9 | PerCP-Cy5.5 | Monocytes |
| CD127 | HIL-7R-M21 | BB790 | ILC phenotyping |
| NKp46 | 9E2/NKp46 | PE | NK phenotyping |
| CRTh2 | BM16 | PE-CF594 | ILC phenotyping |
| CD33 | WM53 | PE-Cy5 | Granulocyte/monocyte exclusion |
| c-kit | 104D2 | PE-Cy5.5 | ILC phenotyping |
| NKG2A | Z199 | PE-Cy7 | NK phenotyping |
| CD337/Nkp30 | AF29–4D12 | APC | NK phenotyping |
| NKG2C | 134,591 | Ax700 | NK phenotyping |
| CD161 | HP-3G10 | APC-Fire750 | MAIT cells/ILC phenotyping |