KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
|
| ||
| Mouse monoclonal anti-c-Myc | Santa Cruz Biotechnology | Cat# sc-40; RRID: AB_2857941 |
| Rabbit polyclonal anti-BRD2 | Bethyl Laboratories | Cat# A302–583A; RRID: AB_2034829 |
| Rabbit polyclonal anti-BRD3 | Bethyl Laboratories | Cat# A302–368A; RRID: AB_1907251 |
| Rabbit polyclonal anti-BRD4 | Bethyl Laboratories | Cat# A301–985A-M; RRID: AB_2631450 |
| Rabbit monoclonal anti-CDK9 (C12F7) | Cell Signaling Technology | Cat# 2316; RRID: AB_2291505 |
| Rabbit monoclonal anti-GAPDH (14C10) | Cell Signaling Technology | Cat# 3683; RRID: AB_1642205 |
| HRP conjugated | ||
| Horse anti-mouse HRP conjugated | Cell Signaling Technology | Cat# 7076S; RRID: AB_330924 |
| Goat anti-rabbit HRP conjugated | Cell Signaling Technology | Cat# 7074P2; RRID: AB_2099233 |
| Annexin V-FITC | BD Biosciences | Cat# 556547; RRID: AB_2869082 |
|
| ||
| Biological Samples | ||
|
| ||
| Patient-derived bone marrow samples | Jerome Lipper Multiple Myeloma Center – Dana Farber Cancer Institute | N/A |
|
| ||
| Chemicals, Peptides, and Recombinant Proteins | ||
|
| ||
| Propidium Iodide staining solution | BD Biosciences | Cat# 556463 |
| dBET6 | J Bradner’s lab | N/A |
| MZ-1 | Tocris Bioscience | Cat# 61545 |
| ARV-771 | MedChemexpress | Cat# HY-100972 |
| JQ1 | J. Bradner’s lab | N/A |
| Bortezomib | Thermo Fisher Scientific | Cat# 507419 |
| Thal-SNS-032 | N Gray’s lab | N/A |
| HM30181 | EMD Millipore | Cat# 533794 |
| Human recombinant interleukin 6 (IL6) | Thermo Fisher Scientific | Cat# 10395HNAE25 |
| Beetle Luciferin, Potassium Salt | Promega | Cat# E1605 |
| CellTiter-Glo Luminescent Cell Viability Assay | Promega | Cat# G7572 |
|
| ||
| Critical Commercial Assays | ||
|
| ||
| Blood & Cell Culture DNA Midi Kit | QIAGEN | Cat# 13343 |
| Blood & Cell Culture DNA Maxi Kit | QIAGEN | Cat# 13362 |
| QIAquick Gel Extraction Kit | QIAGEN | Cat# 28706 |
| QIamp DNA Mini Kit | QIAGEN | Cat# 51304 |
|
| ||
| Deposited Data | ||
|
| ||
| Gene Expression Omnibus | GSE162205 | N/A |
|
| ||
| Experimental Models: Cell Lines | ||
|
| ||
| MM.1S | ATCC | Cat# CRL-2974 |
| RPMI-8226 | DSMZ | Cat# ACC-538 |
| OPM-1 | Teru Hideshima, Anderson Lab, DFCI | N/A |
| OPM-2 | DSMZ | Cat# ACC-50 |
| JJN3 | DSMZ | Cat# ACC-541 |
| L363 | DSMZ | Cat# ACC-49 |
| AMO-1 | DSMZ | Cat# ACC-538 |
| OCI-My5 | Ontario Cancer Institute (OCI); Toronto; Canada | N/A |
| KMS-20 | JCRB | JCRB1196 |
| KMS-26 | JCRB | JCRB1187 |
| KMS-27 | JCRB | JCRB1188 |
| LP-1 | DSMZ | Cat# ACC-41 |
| HS27A | ATCC | Cat# 50188912FP |
| OPM-2.shRNA.CRBN and non-targeting control | K Stewart’s lab | N/A |
| KMS11.shRNA.CRBN and non-targeting control | K Stewart’s lab | N/A |
| MM.1S CRBN−/−(CRISPR-knockout) | W Kaelin’s lab | N/A |
| MM.1S-Cas9+ | B Ebert’s lab | N/A |
| KMS11-Cas9+ | Broad Institute, MIT | N/A |
|
| ||
| Experimental Models: Organisms/Strains | ||
|
| ||
| NOD.Cg-Prkdcscid Il2rgtm1Wjl/SzJ | The Jackson Laboratory | Cat# 005557 |
|
| ||
| Oligonucleotides | ||
|
| ||
| List of primers – see Table S1 | IDT | N/A |
| List of sgRNAs for single-gene knock-out – see Table S2 | IDT | N/A |
|
| ||
| Recombinant DNA | ||
|
| ||
| lentiCas9-Blast | Addgene | RRID: Addgene_52962 |
| lentiGuide-Puro | Addgene | RRID: Addgene_52963 |
| psPAX2 | Addgene | RRID: Addgene_12260 |
| pMD2.G | Addgene | RRID: Addgene_12259 |
| GeCKO v2 human sgRNA library | Addgene | Zhang Lab, Broad Institute of MIT and Harvard |
| Lenti dCAS9-VP64_Blast | Addgene | RRID: Addgene_61425 |
| pLVX-hyg-sgRNA1 | Takara | Cat# 632630 |
| Brunellohuman sgRNA library | Addgene | RRID: Addgene_73179 |
| Human CRISPR Activation Pooled sgRNA Library (Calabrese library) | Addgene | RRID: Addgene_61425 |
|
| ||
| Software and Algorithms | ||
|
| ||
| MAGeCK | Li et al., 2014 | https://sourceforge.net/projects/mageck/ |
| PRISM 6 | GraphPad | https://www.graphpad.com |
| FlowJo V9.7.6 | Tree Star | https://www.flowjo.com/ |
| Biorender | https://www.biorender.com/ | |